Effect of small interfering RNA on expression ofβ2 M in pre-differentiated bone marrow mesenchymal stem cells
10.3969/j.issn.1000-4718.2014.08.013
- VernacularTitle:siRNA对预分化骨髓间充质干细胞β2M表达的影响
- Author:
Bing DAI
;
Shiyang FAN
;
Long CHEN
;
Haidong JIN
;
Jianwu CAI
;
Jun PAN
- Publication Type:Journal Article
- Keywords:
Small interfering RNA;
Beta2-microglobulin;
Bone marrow mesenchymal stem cells
- From:
Chinese Journal of Pathophysiology
2014;(8):1410-1414
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]AIM:TostudytheeffectofsmallinterferingRNA(siRNA)ontheexpressionofbeta2-microglo-bulin (β2M) in pre-differentiated bone marrow mesenchymal stem cells (BMSCs).METHODS: The β2M siRNA was transfected into the pre-differentiated BMSCs with Lipofectamine 2000.BMSCs were divided into transfection group , blank control group and negative control group .The expression of β2 M at mRNA and protein levels was determined by real-time qPCR, Western blotting and laser confocal microscopy .The productions of aggrecan and type II collagen in pre-differentia-ted BMSCs were determined by toluidine blue staining and type Ⅱcollagen immunofluorescence .RESULTS:The results of real-time qPCR, Western blotting and laser confocal microscopy showed that siRNA successfully inhibited the expression ofβ2 M at mRNA and protein levels in the pre-differentiated BMSCs .The results of toluidine blue and type Ⅱcollagen im-munofluorescence staining showed that siRNA does not affect the productions of aggrecan and type Ⅱ collagen in the pre-differentiated BMSCs .CONCLUSION:siRNA targeting β2 M reduces the expression of β2 M in the pre-differentiated BM-SCs and does not affect the chondrocyte characteristics of pre -differentiated BMSCs .
