Role of Toll-like receptor 4 in mitigation of inflammatory responses following myocardial ischemia-reperfusion injury by sufentanil post-conditioning in rats
10.3760/cma.j.issn.0254-1416.2014.05.031
- VernacularTitle:Toll样受体4在舒芬太尼后处理减轻大鼠心肌缺血再灌注损伤后炎性反应中的作用
- Author:
Lixia NIE
;
Yujie MENG
;
Baojiang LIU
- Publication Type:Journal Article
- Keywords:
Toll-like receptor 4;
Sufentanil;
Myocardial reperfusion injury;
Inflammation
- From:
Chinese Journal of Anesthesiology
2014;34(5):634-636
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of Toll-like receptor 4 (TLR4) in mitigation of inflammatory responses following myocardial ischemia-reperfusion (I/R) injury by sufentanil postconditioning in rats.Methods Thirty Wistar rats were randomly divided into 3 groups (n =10 each):sham operation group (S group); I/R group; sufentanil postconditioning group (SP group).Myocardial I/R was produced by temporary ligation of left anterior descending branch of coronary artery for 30 min followed by 120 min reperfusion.In group SP,sufentanil 0.6 μg/kg was injected intravenously at 5 min before reperfusion.The myocardial specimens and blood samples were taken at the end of 120 min reperfusion for determination of the interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) concentrations and TLR4 content (by ELISA).Results Compared with S group,the levels of TLR4,IL-6 and TNF-α were significantly increased in the other two groups.Comparedwith I/R group,the levels of TLR4,IL-6 and TNF-α were significantly decreased in group SP.Conclusion The mechanism by which sufentanil postconditioning mitigates inflammatory responses following myocardial I/R injury may be related to downregulation of TLR4 expression in myocardial tissues of rats.
