Dynamic expression of Lhx8 in nerve growth factor induced hippocampal neuroregeneration
10.3969/j.issn.0529-1356.2014.04.001
- VernacularTitle:神经生长因子促进海马胆碱能神经再生过程中Lhx8的动态表达
- Author:
Haoming LI
;
Peipei ZHU
;
Guohua JIN
;
Jinhong SHI
;
Linqing ZOU
;
Meiling TIAN
;
Xin YI
;
Jianbing QIN
- Publication Type:Journal Article
- Keywords:
Lhx8;
Hippocampus;
Nerve growth factor;
Cholinergic nerve;
Regeneration;
Real-time PCR;
Rat
- From:
Acta Anatomica Sinica
2014;(4):441-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the nerve growth factor ( NGF ) induced hippocampal neuroregeneration and homeobox gene Lhx 8.Methods Seventy-two SD rats were divided into control group , transected group, NGF group, transected combined with NGF group after right fimbria-fornix transection and NGF intracerebroventricular injection . Real-time PCR and Western blotting were applied to detect the gene and protein expression of Lhx8 in each group.The choline acetyltransferase ( ChAT)/Lhx8 double labeled cells in subgranular zone ( SGZ) of hippocampus in each group were detected by immunofluorescence .Results The expression of Lhx8 gene and protein in the transected , NGF group and especially in the transected combined with NGF group was obviously higher than in the control group .The number of ChAT/Lhx8 double labeled cells in the NGF group and the transected combined with NGF group was obviously more than in the control group and transected group . Conclusion The hippocampal neuroregeneration which induced by NGF intracerebroventricular injection was associated with the higher expression of Lhx8.
