Effect on Discornin Tablets of Nuclear Transcription Factor NF-κBp65 in RSC-364 Cells
10.11842/wst.2014.07.031
- VernacularTitle:薯蓣皂苷片对RSC-364细胞核转录因子-κBp65表达的影响
- Author:
Yina DUAN
;
Mingjuan WANG
;
Suhong KONG
;
Yufeng GAO
;
Xiurong ZHAO
;
Hongru SONG
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis;
Discornin Tablets;
rat fibroblast-like synovial cell line;
NF-κB inhibitory protein;
nuclear transcription factor κB
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(7):1625-1628
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the influence of Discornin Tablets on activation nuclear transcription factor NF-κBp65 of rheumatoid arthritis (RA) cell model as well as the expression of MMP-9, VEGF and tumor necrosis factor-α (TNF-α). Interleukin-17 (IL-17) and TNF-α were used for stimulating RSC-364 cells. Discornin Tablets at different concentrations were used for intervention. The influence of Discornin Tablets in different concentrations on cell viability was detected by MTT method. Expressions of NF-κBp65 and its inhibitory protein (IκB-α) in each group were detected by western blot method. Changes in VEGF, MMP-9 and TNF-α protein levels in cell broth supernatant were checked by ELISA. The results showed that Discornin Tablets can promote the expression of κB inhibitory pro-tein, reduce the high expression of NF-κB protein level, and inhibit the cellular secretion of VEGF, MMP-9 and TNF-α. It was concluded that Discornin Tablets had negative regulation effect on nuclear transcription factor κB of RSC-364 cells. It can increase the expression of IκB-α, as well as reduce the secretion of inflammation factors and blood vessel newborn factors. It suggested that Discornin Tablets may have the potential regulation effect on RA.
