Changes of endogenous cystathionine-β-synthase/hydrogen sulfide system in PC12 cells injury induced by rotenone
10.3969/j.issn.1001-1978.2014.10.010
- VernacularTitle:胱硫醚-β-合酶/硫化氢系统在鱼藤酮诱导PC12细胞损伤中的变化
- Author:
Yan XU
;
Na MA
;
Shuyong DENG
;
Jinquan WANG
;
Jianqiang FENG
;
Jinlan MENG
- Publication Type:Journal Article
- Keywords:
rotenone;
Parkinson′s disease;
PC12 cells;
hydrogen sulfide;
cystathionine-β-synthase;
oxi-dative stress
- From:
Chinese Pharmacological Bulletin
2014;(10):1372-1376
- CountryChina
- Language:Chinese
-
Abstract:
Aim To research dynamically the changes of endogenous cystathionine- β-synthase/hydrogen sul-fide system in PC12 cells injury induced by rotenone. Methods Rotenone-induced injury in PC12 cells ( characteristic of dopaminergic neurons) was used as a PD cell model. The expression of CBS was evaluated by Western blot. Intracellular CBS activity and H2 S production were detected by Methylene blue spectro-phot-ometric method. The viability of PC12 cells was measured by CCK-8 assay. GSH detection kit was used to detect the intracellular GSH content. Results In the groups of 6 and 12 hours, the expression and activ-ity of CBS were elevated, and the production of H2 S was increased. In the groups of 24 and 48 hours, CBS expression and activity were significantly decreased, and the amount of H2 S was significantly reduced. Ap-plication of 1. 5 μmol·L-1 rotenone for different time (6-48h) could decrease the cell viability and intra-cellular GSH contents in a time-dependent manner. Conclusions The expression and activity of endoge-nous CBS, stimulated by rotenone, are elevated firstly and then decreased. The generation of H2 S, stimulated by rotenone, is increased and then reduced significant-ly, which may be related to PC12 cells against oxida-tive stress damage induced by rotenone.
