Changes in Fingerprint Pattern of Compound Granatum Berberine Capsules before and after Irradiation
- VernacularTitle:复方石榴皮小檗碱胶囊辐照前后指纹模式变化研究
- Author:
Zhenwei ZHANG
;
Jixi QIU
;
Guoqing LI
;
Xinmin ZHANG
;
Lei SHI
- Publication Type:Journal Article
- Keywords:
Compound granatum berberine capsules;
Irradiation;
Fingerprint pattern;
Comparative study
- From:
China Pharmacist
2014;(9):1480-1483
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the component changes in compound granatum berberine capsules after the radioactive ray ir-radiation sterilization. Methods: The chromatographic fingerprints were obtained on a Diamonsil C18 analytical column ( 250 mm × 4. 6 mm,0. 5 μm) with solvent system composed of acetonitrile-0. 033 mol·L-1 potassium dihydrogen phosphate, the flow rate was 1. 0 ml·min-1 and the detection wavelength was set at 265nm, the column temperature was maintained at 30℃, and the injection vol-ume was 10 μl. Results:Totally 10 co-possessing peaks were selected as the fingerprint peaks for compound granatum berberine cap-sules, and the fingerprints exhibited promising separation for each peak and revealed the fingerprint information according to the techni-cal requirements of fingerprints for Chinese traditional medicines. Most components were with different degrees of reduction, and some peaks even lost after the irradiation. Conclusion:The main ingredients show no statistically significant change after irradiation ( P>0. 05), suggesting the sterilization method is suitable for the preparation.
