Effect of ginkgolide B on expression of Foxg1 gene and proliferation of cells in brain tissue of newborn rats with hypoxic-ischemic brain damage
10.3760/cma.j.issn.2095-428X.2014.10.015
- VernacularTitle:银杏内酯B对缺氧缺血性脑损伤新生大鼠脑组织Foxg1基因表达及细胞增殖的影响
- Author:
Guohui NIU
;
Jun WANG
;
Fengwei SHANG
;
Dengna ZHU
;
Xiaoli ZHANG
- Publication Type:Journal Article
- Keywords:
Hypoxic-ischemic brain damage;
Ginkgolide B;
Foxg1 gene
- From:
Chinese Journal of Applied Clinical Pediatrics
2014;29(10):777-780
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of ginkgolide B (GB) on mRNA expression of foxgl and proliferation of cells in brain tissue of newborn rats with hypoxic-ischemic brain damage (HIBD).Methods A total of 128 clean 7-day-old healthy SD rats were randomly divided into sham operation group,the model group,the low-GB dose and the high-GB dose treatment groups.Classic Rice method were used to establish HIBD models in the latter 3 groups.Four hours after operation,and GB in dose of 5 mg/kg and 10 mg/kg was given to rats in the low and the high dose treatment groups by intraperitoneal injection postoperatively,once a day for 5 days,while sham operation and model groups were treated with equal physiological saline.All groups were respectively sacrificed on 3 d,7 d,14 d,28 d respectively.Quantitative real-time fluorescent polymerase chain reaction was employed to detect expression of Foxg1 gene.Then the number of 5-bromodeoxyuridine positive cell in subgranular zone was investigated by immunolluorescent stairning.Results The Foxg1 mRNA expression was observed 3 days after HIBD,peaked on 7th day,and then declined gradually; the levels of Foxg1 mRNA in the 2 treatment groups were higher than that of the HIBD group (all P ＜ 0.01) ; The expression of Foxgl at 7 d,14 d,28 d,in high-dose group were higher than those in the low-dose group (all P ＜ 0.01).The number of 5-bromodeoxyuridine positive cell was increased after HIBD,and the levels in the low-and the high-dose treatment groups were all higher than that of the model group (all P ＜ 0.05) ; the number of positive cell in high-dose treatment groups were higher than that in the low-dose treatment groups (P ＜ 0.05).Conclusions GB can promote the expression of Foxg1 gene and improve the proliferation of cells in Brain tissue after HIBD,which shows more significant efficacy in high-dose group than in low-dose group.
