Determination of Warfarin in Human Plasma by HPLC
- VernacularTitle:HPLC法测定人血浆中华法林药物浓度
- Author:
Xiaohui HUANG
;
Ping YANG
;
Yan LIU
;
Huaiping TIAN
;
Jian ZHANG
- Publication Type:Journal Article
- Keywords:
Warfarin;
Drug concentration detection;
HPLC
- From:
China Pharmacist
2014;(2):199-201
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a simple HPLC method for the determination of warfarin in human plasma and analyze the re-sults of 100 monitoring reports. Methods:Warfarin was extracted from the plasma samples by acetonitrile. The extract was separated on a ZORBAX Eclipse XDB-C8 column (150 mm × 4. 6 mm, 5 μm)with a mobile phase consisting of methanol and 0. 1% acetic acid (65∶35) at flow rate of 1. 0 ml·min-1 and detected by a diode array detector. The detection wavelength was at 308nm and the sample size was 20 μl. Results:The calibration curve was linear within the range of 0. 1-5. 12μg·ml-1 . The average recovery and RSD was ranged from 85. 0% to 102. 3% and 1. 2% to 3. 9%(n=6), respectively. Inter-and intra-day RSD were 1. 1%-1. 8% and 1. 6%-2. 9%, respectively. Conclusion:The proposed method is rapid, convenient and accurate in the detection of warfarinin concentration in plasma.
