Establishment of mice breast cancer model in BALB/c mice with 4T1 cells
10.3969/j.issn.1000-484X.2014.06.016
- VernacularTitle:BALB／c小鼠乳腺癌4 T1细胞株移植模型的建立
- Author:
Li YAN
;
Li LI
;
Fang HAO
;
Wei SHEN
;
Linlei ZHANG
;
Hao GUO
- Publication Type:Journal Article
- Keywords:
Breast-cancer;
BALB/c-mice;
4T1-cells
- From:
Chinese Journal of Immunology
2014;(6):794-796
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a model of mice breast cancer of 4T1 cells in BALB/c mice and choose the best model making method.Methods:Ninety mice were divided into three groups randomly ,with 30 in each group injected by 4T1 cells suspension of 1×106 ml-1 ,1×107 ml-1 ,1×108 ml-1 respectively.Each group of mice were randomly divided into two groups which were inoculated on the chest wall and lateral abdominal wall respectively.Tumor formation time ,tumor growth rate and the 8 week survival rate in each group were compared ,and pathological character was observed by C-erbB-2 immunohistochemistry staining.Results:Tumor growth rate in cells suspension of 1×107 ml-1 was high and grow steadily.Tumor growth rate wasn′t correlative with 4T1 cells injection in different parts.The result of C-erbB-2 immunohistochemistry staining was positive.Conclusion: Injection of 4T1 cells suspension with 1 ×107 ml-1 is the best way to male tumor model in three suspension.
