Inhibition Effect of Water-solubility Nipponica Saponin on NF-κB Pathway of Rheumatoid Cellular Model
10.11842/wst.2014.06.033
- VernacularTitle:穿山龙水溶性总皂苷对RA细胞模型及其下游因子的影响
- Author:
Yina DUAN
;
Mingjuan WANG
;
Jiaqi YANG
;
Yufeng GAO
;
Guangming CHENG
;
Shubin MI
;
Hongru SONG
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis;
water-solubility nipponica saponin;
rat fibroblast-like synovial cell line;
NF-κBp65
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(6):1396-1400
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the influence of water-solubility nipponica saponin on activation of TNF-α+IL-17-induced rat fibroblast-like synovial cell line RSC-364 cellular model nuclear transcription factor NF-κB pathway as well as TNF-α, IL-1, ICAM-1, MMP-2, MMP-3 secretion. IL-17+ TNF-α were used for stimulating RSC-364 to establish rheumatoid arthritis (RA) cellular model. Water-solubility nipponica saponin in different con-centrations was used for intervention. The influence of water-solubility nipponica saponin in different concentrations on cell viability was detected by semi-quantitative RT-PCR method. Changes in the level of TNF-α, IL-1, ICAM-1, MMP-2, and MMP-3 of culture supernatant were detected by ELISA. The results showed that the activation of NF-κB p65 in RSC-364 stimulated by TNF-α+ IL-17 can be inhibited by water-solubility nipponica saponin ac-cording to its concentration. It improved IκB-α expression, and inhibited TNF-α, IL-1, ICAM-1, MMP-2 and MMP-3 secretion. It was concluded that water-solubility nipponica saponin can inhibit the activation of NF-κB pathway, hinder the secretion and activation of multiple downstream genes, which may be its effect in inhibiting syn-ovial inflammation in RA.
