Transcriptional Analysis of Nod-Like Receptors in a Mouse Model of Experimental Autoimmune Uveitis.
10.3341/jkos.2015.56.1.99
- Author:
Hyeong Min KIM
1
;
Tae Wan KIM
;
Hyun Jeong JEONG
;
Jin Suk RYU
;
Mee Kum KIM
;
Won Ryang WEE
;
Joo Youn OH
Author Information
1. Department of Ophthalmology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea. jooyounoh77@gmail.com
- Publication Type:Original Article
- Keywords:
Experimental autoimmune uveitis;
NLRP3;
Nod-like receptors
- MeSH:
Animals;
Carrier Proteins;
Humans;
Immunization;
Inflammation;
Injections, Intraperitoneal;
Injections, Subcutaneous;
Interleukin-1beta;
Leucine;
Mice*;
Pertussis Toxin;
Polymerase Chain Reaction;
Retinaldehyde;
Reverse Transcription;
Uveitis*
- From:Journal of the Korean Ophthalmological Society
2015;56(1):99-103
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the transcription pattern of Nod-like receptors (NLRs), the intracellular sensors, to detect danger signals in murine eyes with experimental autoimmune uveitis (EAU). METHODS: EAU was induced in B6 (C57BL/6) mice by subcutaneous injection of human interphotoreceptor retinoid binding protein and intraperitoneal injection of pertussis toxin. At 1, 2, and 3 weeks post-immunization, the eyeballs were extracted and subjected to histological and molecular assays using real-time reverse transcription polymerase chain reaction. RESULTS: The levels of nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain 1 (NLRP1), NLRP3, nucleotide-binding oligomerization domain-containing protein 1 (NOD1), and NOD2 transcripts were increased at 2 weeks and gradually reduced thereafter. Notably, NLRP3 showed the highest expression in the eyes with EAU. Similarly, the transcript level of pro-inflammatory cytokine, interleukin-1beta, increased and reached a peak at 2 weeks post-immunization. The retinal structure was severely damaged by inflammation at 3 weeks post-immunization. CONCLUSIONS: Among NLRs, NLRP3 may induce inflammation in eyes after EAU immunization.
