Simultaneous Determination of Three Flavonol Glycosides in the Stems and Leaves of Tribulus terrestris L . by HPLC
10.11842/wst.2014.05.019
- VernacularTitle:HPLC法同时测定蒺藜茎叶中3个黄酮醇苷的含量
- Author:
Haishui GUI
;
Tonghua LIU
;
Lingling QIN
;
Pengshou LI
;
Chunna LI
;
Yangyang LIU
;
Tunhai XU
- Publication Type:Journal Article
- Keywords:
Tribulus terrestris L.;
stems and leaves;
flavonoids;
HPLC;
content determination
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(5):1055-1059
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, three higher content of flavonol glycosides from the stems and leaves of Tribulus terrestris L. were determined by reversed phase HPLC. The results showed that there was good liner relationship between the peak areas and the sample concentration at the ranges of 0.011 2~0.280 0 μg (r=0.999 6), 0.064 8~2.592 0 μg (r=0.999 8) and 0.018 4~0.460 0 μg (r=0.999 8) for isorhamnetin-3-O-β-D-gentiobioside-7-O-β-D-glucoside, quercetin-3-O-β-D-gentiobioside and isorhamnetin-3-O-β-D-gentiobioside respectively. The verage recovery rates (n=9) of three flavonol glycosides compounds were 100.15%(RSD=1.32%), 100.02% (RSD=1.14%), 99.77% (RSD=1.16%), respectively. This method is considered to be simple, accurate, stable, good precision and reproducibility, which is available for the quality control and evaluation of T. terrestris L.
