Determination of catalpol in dried rehmannia root and Taohong Siwu Decoction with high performance liquid chromatography
- Author:
Chunyan GUO
;
Xuemei BAI
;
Wanming ZHANG
;
Zhibao WANG
;
Yurong FU
- Publication Type:Journal Article
- From:
Journal of Integrative Medicine
2004;2(2):135-7
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determinate the catalpol contents in dried rehmannia root and Taohong Siwu Decoction containing rehmannia root with high performance liquid chromatography (HPLC). METHODS: Catalpol was separated on a YWG-C18 column using water-acetonitrile (99.4:0.6) as mobile phase and detective wavelength at 210 nm. RESULTS: The linear curve of tested catalpol concentration within the range of 0.0536-5.3600 microg/microl was ideal (n=5, r=0.999 7). The average recovery rate of the dried rehmannia root and Taohong Siwu decoction was 98.7% (RSD=0.48%) and 98.2% (RSD=1.29%) respectively. CONCLUSION: HPLC method is accurate and valuable for the quality control of Radix Rehmanniae and Taohong Siwu Decoction.
