Identification of radix et rhizoma clematidis and its adulterants using DNA barcoding.
- Author:
Shanshan FENG
;
Sihao ZHENG
;
Yakang LI
;
Linfang HUANG
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2014;49(2):260-6
- CountryChina
- Language:Chinese
-
Abstract:
This study provides the candidate sequences in the identification of Radix et Rhizoma Clematidis and its adulterants using DNA barcoding. We amplified and sequenced the region psbA-trnH, with the data of 284 sequences from GenBank, the differential intra- and inter-specific divergences, genetic distance, barcoding gap were used to evaluate five barcodes, and the identification efficiency was assessed using BLAST1 and Nearest Distance methods. The results showed that psbA-trnH barcodes performed high identification efficiency and inter-specific divergences among the five different DNA barcodes. Analysis of the barcoding gap and NJ tree showed psbA-trnH was superior to other barcodes. Based on the identification and PCR amplification efficiency, psbA-trnH can be the ideal barcode to identify Radix et Rhizoma Clematidis and its adulterants accurately.
