A mycoplasma lipopeptide induces hemeoxygenase-1 expression via TLR2, 6/c-Src/PI3 K pathway in monocytes
10.3969/j.issn.1000-484X.2014.05.003
- VernacularTitle:支原体脂肽经TLR2，6／c-Src／PI3 K通路诱导单核细胞表达血红素氧合酶-1
- Author:
Xiaoxing YOU
;
Xiaohua MA
;
Liangzhuan LIU
;
Yanhua ZENG
;
Cuiming ZHU
;
Jun HE
;
Chuanhao JIANG
;
Yimou WU
- Publication Type:Journal Article
- Keywords:
Macrophage-activating lipopeptide-2;
Hemeoxygenase-1;
Monocyte
- From:
Chinese Journal of Immunology
2014;(5):587-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the molecular mechanism involved in expression of hemeoxygenase -1 (HO-1) induced by a macrophage-activating lipopeptide-2 (MALP-2).Methods:THP-1 cells were cultured in vitro and stimulated by MALP-2 for 12 h, expression of HO-1 was detected by Western blot .TLR2 and TLR6 neutralizing antibodies incubation , dominant negative plasmids transfection were used to assess the functional of TLR 2,6 in mediating HO-1 expression.Phosphorylation of c-Src and Akt were detec-ted by Western blot, and c-Src siRNA and PI3K inhibitor LY294002 were used to investigate the role of c-Src and PI3K in HO-1 ex-pression.Results:MALP-2 induced c-Src phosphorylation , and TLR2 and TLR6 neutralizing antibodies , or their dominant negatively plasmids could abrogate this effect .In addition, siRNA of c-Src could decrease the phosphorylation level of Akt , and the PI3K inhibi-tor could inhibit HO-1 expression.Conclusion: MALP-2 can induce THP-1 cells expression of HO-1 through TLR2,6/c-Src/PI3K pathways .
