Regulation effect of complement C5a on interleukin-17 during renal allograft rejection
10.3969/j.issn.1671-8348.2014.11.018
- VernacularTitle:补体C5a对肾移植排斥反应中IL-17产生的调节作用
- Author:
Shujing LI
;
Quanyou ZHENG
;
Gang YUAN
;
Wenqian HUO
;
Keqin ZHANG
- Publication Type:Journal Article
- Keywords:
complement C5a;
interleukin-17;
kidney transplantation;
graft rejection
- From:
Chongqing Medicine
2014;(11):1331-1334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of proinflammatory cytokine interleukin-17(IL-17) and complement cleavage fragment C5a and the regulation effect of C5a on the expression of IL-17 during renal allograft rejection .Methods The frequency of IL-17+ T cell in peripheral blood and the expression of IL-17 in renal tubular epithelial cells (HK2) after C5a stimulation in renal transplant recipients were measured by flow cytometry and the changes of serum C5a level was detected by ELISA ,respectively .Im-munohistochemistry was applied to detect and compare the expression of IL-17 and the deposition of C5b-9 in normal renal tissues and renal tissues with allograft rejection .The difference of IL-17 expression in HK2 cells before and after the recombinant C5a stimulation was detected by immunocytochemistry .Results Both the percentage of IL-17+ T cells and serum C5a levels were sig-nificantly increased after the allogeneic renal transplantation .Compared with normal renal tissues ,both the deposition of C5b-9 and the IL-17 expression in renal tissues with allograft rejection was remarkably up-regulated ,which showed the positive correlation be-tween them .The expression of IL-17 in HK2 was obviously up-regulated by the recombinant C5a stimulation .Conclusion C5a may positively regulate the expression of IL-17 by tubular epithelial cells during the renal allograft rejection .
