Determination of aloin of compound aloe ointment by HPLC
10.3760/cma.j.issn.1673-4246.2014.05.016
- VernacularTitle:HPLC测定复方芦荟软膏中芦荟苷的含量
- Author:
Mingdi ZHANG
- Publication Type:Journal Article
- Keywords:
Aloin;
Compound aoe ointment;
HPLC
- From:
International Journal of Traditional Chinese Medicine
2014;(5):445-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of aloin of compound aloe ointment. Methods HPLC method was used with Agilent Zorbax-C18(250 mm×4.6 mm,5μm)column and the mobile phase consisted of acetonitrile-0.08%Sodium 1-heptanesulfonate (22∶78). The flow rate was 1.0 ml/min,the column temperature was 30 ℃, and the UV detector was set at 355 nm. Results The linear ranges of aloin was 0.037~0.74 mg/ml(r=0.999 8). The average recovery of aloin was 98.7%. Conclusion The method is simple, repeatable and accurate, it can be applied in quantitative determination of aloin in compound aloe ointment.
