CHST13 inhibits the invasive property of human hepatocellular carcinoma cells
10.3760/cma.j.issn.1673-422X.2014.04.022
- VernacularTitle:CHST13抑制肝癌细胞侵袭能力的研究
- Author:
Yanping LI
;
Youxun LIU
;
Hongye MA
;
Ning WANG
;
Xu ZHANG
- Publication Type:Journal Article
- Keywords:
Sulfotransferases;
Carcinoma,hepatocellular;
Neoplasm metastasis
- From:
Journal of International Oncology
2014;41(4):314-318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the association between chondroitin-4-O-sulfotransferase-3 (CHST13) and hepatoma metastasis by the research of differential expression of CHST13 in MHCC97-H and MHCC97-L human hepatocarcinoma cell lines,which have high and low metastatic potential,respectively,and to confirm novel target of hepatoma metastasis and anti-tumor therapy.Methods The differential expressions of CHST13 in MHCC97-H and MHCC97-L human hepatocarcinoma cell lines were analyzed by real-time PCR,Western blot.CHST13 was silenced using RNA interference approach to detect the invasive ability in vitro and tumorigenicity in vivo in MHCC97-L cells.Results The expression of CHST13 was different in MHCC97-L cells,as compared to those in MHCC97-H cells.Knockdown of CHST13 expression enhanced MHCC97-L cells invasion and tumorigenicity both in vitro (t =2.8,P =0.005) and in vivo (t =2.5,P =0.01).The quantity of cells which crossed basement membrane increased [(30 ± 3):(14 ± 2)],and the average weight of tumor increased [(0.9 ± 0.10) g:(0.5 ± 0.06) g].Conclusion The differential expressions of CHST13 in human hepatocarcinoma cell lines correlate with tumor invasion and tumorigenicity,and it is expected to be a novel target for the treatment of hepatocellular carcinoma.
