Simultaneous Determination of Eprosartan/Hydrochlorothiazide Tablets by HPLC
- VernacularTitle:HPLC法同时测定依普罗沙坦/氢氯噻嗪片的两组分含量
- Author:
Yang LI
;
Xiangyang XIE
;
Chen CHEN
;
Liang HAN
;
Xiangru LIAO
;
Ying CHEN
- Publication Type:Journal Article
- Keywords:
Eprosartan;
Hydrochlorothiazide;
Content determination;
HPLC
- From:
China Pharmacist
2014;(4):582-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the simultaneous determination of eprosartan/hydrochlorothiazide tablets. Methods:Isocratic separation was achieved on a Phenomenex C18 column(250 mm × 4. 6 mm, 5 μm) using the mobile phase com-posed of 0. 5% formic acid-acetonitrile(60∶40, pH 2. 80). The flow rate was 1. 0 ml·min-1, the detection wavelength was 272nm, the column temperature was 30℃ and the injection volume was 20μl. Results:The linearity between peak area and concentration was observed within the range of 60. 0-1 200. 0 mg·L-1(r=0. 999 9) for eprosartan and 1. 25-25. 00 mg·L-1(r=0. 999 9) for hydro-chlorothiazide. The mean recovery of eprosartan and hydrochlorothiazide was 100. 02%(RSD=0. 35%, n=9) and 97. 93%(RSD=1. 54%, n=9), respectively. Conclusion:The method is simple, sensitive and accurate, and can be applied in the determination of eprosartan/hydrochlorothiazide tablets.
