Effects of matrine on apoptosis and related protein expression in human medulloblastoma D341 cells
10.3969/j.issn.1000-4718.2014.04.010
- VernacularTitle:苦参碱对人髓母细胞瘤 D341细胞凋亡及相关蛋白表达的影响
- Author:
Kaiyu ZHOU
;
Tianming MAO
;
Xi CHEN
;
Yongkang LUO
- Publication Type:Journal Article
- Keywords:
Matrine;
Medulloblastoma;
Bax protein;
Bcl-2 protein;
Serine/threonine kinase Akt
- From:
Chinese Journal of Pathophysiology
2014;33(4):629-634
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate matrine-induced apoptosis of human medulloblastoma D 341 cells and the ex-pression of Bax , Bcl-2, serine/threonine kinase Akt and phosphorylated Akt ( p-Akt) in vitro.METHODS: D341 cells were divided into experimental groups ( added with matrine at different concentrations ) and control group ( under the same conditions without matrine).The proliferation of D341 cells was analyzed by CCK-8 assay.Apoptosis was detected by An-nexin V-FITC/PI double staining and the expression of Bax , Bcl-2, Akt and p-Akt was detected by Western blotting .RE-SULTS:Matrine significantly inhibited the proliferation of D 341 cells and increased the apoptosis in a dose-and time-de-pendent manner .The cell apoptosis was characterized by chromatin condensation with margination of chromatin to the nu -clear membrane , increased when and larger cytoplasmic vacuoles , and formation of apoptotic body after treatment with ma-trine.The expression of Bax increased , while the expression of Bcl-2 and p-Akt decreased when the drug concentration gradually increased .CONCLUSION:Matrine induces the apoptosis of human medulloblastoma D 341 cells in vitro by acti-vation of Bax, down-regulation of Bcl-2 and reduction of p-Akt expression level in the PI3K/Akt signaling pathway.
