Effect of recombinant Mycobacterium tuberculosis heat shock protein 10 on proliferation of human osteoblasts and regulation of bone metabolism
10.3969/j.issn.2095-4344.2014.11.005
- VernacularTitle:重组结核杆菌热休克蛋白10对人成骨细胞增殖及骨代谢的调节
- Author:
Yuanyu ZHANG
;
Xia LIU
;
Kun LI
;
Yongrong GUO
;
Jingping BAI
- Publication Type:Journal Article
- Keywords:
osteoblasts;
cel proliferation;
heat-shock proteins;
alkaline phosphatase
- From:
Chinese Journal of Tissue Engineering Research
2014;(11):1665-1671
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Mycobacterium tuberculosis heat shock protein 10 (r-Mt cpn10) is one of the main factors that cause bone tuberculosis dissolution and absorption as wel as inhibits the proliferation of osteoblasts. Receptor activator of nuclear factor kappa B ligand and osteoprotegerin are the important factors influencing bone metabolism.
OBJECTIVE:To observe the effect of r-Mt cpn10 on human osteoblast proliferation, alkaline phosphatase secretion, expression of receptor activator of nuclear factor-kappa B ligand mRNA and osteoprotegerin mRNA. METHODS:Human bone marrow stromal cel s were induced to differentiate into osteoblasts, and osteoblasts at passage 3 were cultured with various concentrations of r-Mt cpn10 (0.1, 1, 10 mg/L). Osteoblasts cultured without r-Mt CPN10 were assigned as controls.
RESULTS AND CONCLUSION:MTT assay results showed that, compared with control group, r-Mt cpn10 at different concentrations inhibited osteoblast proliferation and alkaline phosphatase secretion (P<0.05). RT-PCR analysis showed that, r-Mt cpn10 at different concentrations increased receptor activator of nuclear factor-kappa B ligand mRNA expression (P<0.01), and inhibited osteoprotegerin mRNA expression in a concentration-dependent manner (P<0.01). 10 mg/L r-Mt cpn10 exhibited the strongest effect (P<0.01). The r-Mt cpn10 can inhibit osteoblast proliferation and alkaline phosphatase activity, and it may influence bone metabolism by regulating the expression of receptor activator of nuclear factor-kappa B ligand mRNA and osteoprotegerin mRNA.
