The study of acute hypobaric hypoxia on variation biochemical index of renal tissue in rats
10.3969/j.issn.1671.7856.2014.003.007
- VernacularTitle:急性低压缺氧对大鼠肾组织中生化指标变化的研究
- Author:
Zhe WANG
;
Guangjin GUO
;
Min XIONG
;
Tianhui NIU
- Publication Type:Journal Article
- Keywords:
Acure hypobaric hypoxia;
Superoxide dismutase;
Malondialdehyde;
Glutathione;
Catalase;
Endothelin;
Tumor necrosis factor alpha
- From:
Chinese Journal of Comparative Medicine
2014;(3):30-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of superoxide dismutase ( SOD ) , malondialdehyde ( MDA ) , glutathione (GSH), catalase (CAT) and endothelin (ET-1) and tumor necrosis factor alpha (TNF-α) on rat renal tissue under acute hypoxia .Methods 24 male Wistar rats, weight 180~220 g, were randomly divided into control group and acute hypobaric hypoxia group .Acute hypoxia group was divided into 2 groups hypoxia 1 and hypoxia 2, 8 rats for each group.After acute hypobaric hypoxia 10min and 24h, rats were sacrificed.The left removed kidneys were analyzed for biochemical indexes , and the right parts were observed by immunohistochemistry to evaluate the expression level of renal endothelin (ET-1) and tumor necrosis factor alpha (TNF-α).Results After acute hypobaric hypoxia , the activity of SOD of the rats kidney was greatly decreased (P <0.01), CAT activity of hypoxia group 1 was significantly decreased (P <0.01), GSH activity of hypoxia group 2 was significantly decreased (P <0.05), but the MDA content had no obvious change ( P >0.05).The immunohistochemical staining showed that , the expression level of ET-1 and TNF-αwas increased remarkably, but it was reduced after 24 h.Conclusion The obviously decreased activity of SOD , CAT, GSH and significantly increased expression of ET-1 and TNF-α, may be involved in the pathogenesis of renal hypoxic injury .
