Simultaneous Determination of Total Content of p-Hydroxybenzoic Acid plus o-Hydroxybenzoic Acid and p-Hydroxybenzeneacetic Acid in Senecio Scandens Buc
- VernacularTitle:HPLC法同时测定千里光中对羟基苯甲酸和邻羟基苯甲酸的总含量及对羟基苯乙酸的含量
- Author:
Zuojun WANG
;
Ping WEI
;
Hui JIA
;
Guobing SHI
- Publication Type:Journal Article
- Keywords:
Senecio scandens Buch.;
p-Hydroxybenzoic acid;
o-Hydroxybenzoic acid;
p-Hydroxybenzeneacetic acid;
HPLC
- From:
China Pharmacist
2014;(5):767-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To set up an HPLC method for the simultaneous determination of p-hydroxybenzoic acid plus o-hydroxy-benzoic acid and p-hydroxybenzeneacetic acid in Senecio scandens Buch. Methods:The column was a Shiseido( Fine Chemicals) Cap-cell Pak C18 (250 mm × 4. 6 mm, 5 μm) column at the room temperature. The mobile phase was methanol-water-formic acid (13∶87∶0. 5) at a flow rate of 1. 0 ml·min-1 . The detection wavelength was 240nm. Results: p-Hydroxybenzoic acid plus o-hydroxybenzoic acid had a favorable linear relationship within the range of 0. 025-0. 400 mg·ml-1 , the regression equation was Y=5. 94 × 106 X+2.46×104(r=0.9998),theaveragerecoverywas97.59% andRSDwas1.22%. p-Hydroxybenzeneaceticacidhadafavorableline-ar relationship within the range 0.05-0.80 mg·ml-1, the regression equation was Y=4.09 ×106X+1.12 ×104(r=0.999 8), the average recovery was 98. 07% and RSD was 1. 90%. Conclusion:The method is simple, feasible and reproducible. It can be used in the quality control of p-hydroxybenzoic acid plus o-hydroxybenzoic acid and p-hydroxybenzeneacetic acid in Senecio scandens Buch.
