Effects of tobacco extract on the proliferation and HSP70 expression of human periodontal ligament cells
10.3969/j.issn.1001-3733.2014.02.014
- VernacularTitle:烟草浸提液对人牙周膜细胞 HSP70表达的影响
- Author:
Wei LI
;
Dong CHEN
;
Kunyang LI
;
Yun FAN
;
Fangfang YU
- Publication Type:Journal Article
- Keywords:
Human periodontal ligament cells(hPDLCs);
Tobacco;
HSP70
- From:
Journal of Practical Stomatology
2014;(2):211-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of smokeless tobacco extract(ST)on the proliferation and the heat shock protein 70 (HSP70)expression of human periodontal ligament cells(hPDLCs).Methods:hPDLCs were cultured in vitro and identified by im-munohistochemistry(IHC).The cells were stimulated with ST at 0.01 6 -50 g/L respectively for 24 h,the proliferation was examine by MTT assay,HSP70 expression was detected by immunehistochemical staining and Western Blot.Results:ST inhibited the prolifer-ation and increased HSP70 expression in cytoplasm and nucleus at 0.4 -50 g/L dose dependantly.Conclusion:ST may inhibit the proliferation and increase HSP70 expression of hPDLCs in a dose depandant manner.
