Construction of tissue-engineered cartilage with cross-linked sodium hyaluronate as scaffold materials in vitro
10.3969/j.issn.2095-4344.2014.08.008
- VernacularTitle:以交联透明质酸钠为支架体外构建组织工程软骨
- Author:
Shaoying LIU
;
Jianying CHEN
;
Qianqian CHEN
;
Fei LIU
;
Xia LIU
;
Xiqiang ZHU
;
Peixue LING
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
chondrocytes;
hyaluronic acid;
cross-linking reagents
- From:
Chinese Journal of Tissue Engineering Research
2014;(8):1191-1197
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Tissue engineering provides new ideas and approaches for repair of cartilage defects.
OBJECTIVE:To develop a complete set of solutions for construction of tissue engineered cartilagein vitro, with chondrocytes as seed cels and cross-linked sodium hyaluronate as scaffold materials.
METHODS: New Zealand rabbit articular chondrocytes were isolated, counted, and then cultured and passaged to prepare cellsuspension. Toluidine blue staining, RT-PCR and immunocytochemistry were exerted to evaluate the cultured cels. Chondrocytes were seeded and co-cultured with cross-linked sodium hyaluronate scaffold for 21 days. Then, RNA was isolated for RT-PCR, and frozen sections were prepared for morphological observation and immunohistochemistry study.
RESULTS AND CONCLUSION:The chondrocytes could adhere to the cross-linked sodium hyaluronate scaffold and aggregate, growing between fibers or adhering to the scaffold in a monolayer manner. The transcripts of cartilage specific aggrecan gene and colagen type II alpha 1 gene and cartilage specific protein colagen type II were expressed in cel-scaffold complexes to maintain the phenotype of chondrocytes. Cel-scaffold complexes co-cultured in vitro can form cartilage extracelular matrix, by which tissue engineered cartilage is expected to be obtained.
