Expression of specific marker molecules of long-term cultured human dermal papilla cells in vitro
10.3760/cma.j.issn.1671-0290.2013.05.017
- VernacularTitle:体外培养人毛乳头细胞特异性标记物表达的分析
- Author:
Zhidan ZHANG
;
Zhiqi HU
;
Kecheng LI
;
Chuanbo FENG
;
Zehua LI
;
Jindou JIANG
- Publication Type:Journal Article
- Keywords:
Human dermal papilla cells (HDPCs);
Alkaline phosphatase (ALP);
Insulinlike growth factor-1 (IGF-1)
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2013;19(5):377-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of specific marker molecules in hair-inducing activity of long-term cultured human dermal papilla cells (HDPCs) in vitro.Methods After dissected and cultured the HDPCs in vitro,the cells of passages 1 to 8 were used for experiments.The growth appearances of HDPCs in different passages were observed under inverted microscope.To detect the expression of specific marker molecules of long-term cultured HDPCs,the alkaline phosphatase (ALP) activity of the HDPCs was examined,and the specific genes ALP and insulin-like growth factor-1 (IGF-1) expression levels of HDPCs were determined by real-time quantitative PCR.Results After long-term cultured in vitro,the ALP and IGF-1 expression levels of HDPCs gradually decreased in different passages,as well as the display of the aggregated and cartouche growth.The ALP and IGF-1 expression levels of HDPCs in passage 1 was the highest,they were almost about 6.8-fold and 3.5-fold higher than the HDPCs in passage 8.The ALP staining of the HDPCs in passage 1 and passage 2 were evident,but the cells' ALP staining gradually became much weaker than the cells in the previous passages after the long-term cultured in vitro.Conclusions The expression levels of specific marker molecules ALP and IGF-1 of the HDPCs decrease gradually after long-term cultured in vitro,and the higher passage HDPCs lost the special aggregated and cartouche growth appearance,and hence lead to the loss of hair-inducing activity of HDPCs.
