Hypoxic preconditioning up-regulates the activity and gene expressions of glucose transporters (GLUT1 and GLUT3) in cultured rat hippocampal neurons and astrocytes under anoxic condition
- VernacularTitle:低氧预适应上调大鼠海马神经元和星形胶质细胞在急性缺氧时的葡萄糖转运蛋白的活性和基因表达
- Author:
Erfu WANG
;
Xin LI
;
Chunsong JIA
;
Guangwei LIU
;
Haixia FANG
;
Shun YU
- Publication Type:Journal Article
- Keywords:
hypoxic preconditioning;
glucose transport;
neuron;
astrocyte
- From:
Basic & Clinical Medicine
2009;29(12):1273-1276
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of hypoxic preconditioning on the activity and gene expressions of glu-cose transporters in the cultured rat hippocampal neurons and astrocytes under anoxic condition. Methods The cultured rat hippocampal neurons and astrocytes were treated for 6 days by intermittently exposing to hypoxic gas mixture (1% O_2, 10% CO_2, 89% N_2) for20 min each day. 24 h after the last hypoxic exposure, the cells were exposed to anoxic gas mixture (10% CO_2, 90% N_2) for 6 h, and the uptake rate of [~3H]-2-deoxyglucose (2-DG), the levels of glucose transporter GLUT1 and GLUT3 mRNAs and the cell survival rate were examined im-mediately after anoxic exposure. Results Neurons and astrocytes preconditioned with hypoxia showed higher 2-DG uptake rates and increased expressions of GLUT 1 mRNA in the astrocytes and GLUT 1 and GLUT 3 mRNA in the neurons. The preconditioned neurons also showed an increased tolerance to anoxia. Conclusion Hypoxic precon-ditioning up-regulates the activity and gene expressions of glucose transporters of hippocampal neurons and astro-cytes under anoxic condition.
