Study on polymorphism of Brucella melitensis biovar 3 strains isolated from Guangdong province

VernacularTitle:广东地区羊种3型布鲁氏菌基因多态性研究
Author: Jingdiao CHEN ; Jianhui CHEN ; Changwen KE ; Bixia KE ; Meizhen LIU ; Hailing TAN ; Bosheng LI ; Xingfen YANG
Publication Type:Journal Article
Keywords: Brucella melitensis; MLVA; PCR; Subtyping
From: Chinese Journal of Microbiology and Immunology 2013;(10):766-772
CountryChina
Language:Chinese
Abstract: Objective To investigate the polymorphism of Brucella melitensis biovar 3 ( B.melitensis biovar 3) strains isolated from Guangdong province .Methods PCR assays followed by agar-ose gel electrophoresis and capillary electrophoresis based on the multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) were performed to analyze 43 clinic isolates of B.melitensis biovar 3 strains isolated from clinical patients in Guangdong province .Results MLVA typing showed that the simi-larities of the analyzed locus among 43 strains of Brucella ranged from 68.2%to 100%.32 genotypes identi-fied among the isolates were identical (100%similarity).27 out of 43 strains (62.8%) were single geno-types, while the other 16 strains (37.2%) belonged to 5 other genotypes with 2 to 5 strains in each of them . Conclusion B.melitensis biovar 3 isolates showed polymorphism distribution in Guangdong province as in-dicated by MLVA typing analysis .Single-genotype isolates accounted for 62.8% of all studied strains.No predominant genotype was found among all isolates .