The expression of micro RNA130a on rat bone mesenchymal stem cells during chondrogenic differentiation
- VernacularTitle:骨髓间充质干细胞向软骨细胞分化过程中miR130α的表达
- Author:
Jinmei SU
;
Ye JIN
;
Qiang QU
;
Fengchun ZHANG
;
Fulin TANG
- Publication Type:Journal Article
- Keywords:
bone mesenehymal stem cell;
chondrocyte;
differentiation;
microRNA
- From:
Basic & Clinical Medicine
2010;30(5):520-523
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of microRNA130α on rat bone mesenchymal stromal cells(BMSCs)during chondrogenic differentiation.Methods BMSCs were induced to differentiate into chondroeytes by transforming growth factor-β1(TGF-β1)in vitro,immunofluorescence and immunohistochemistry were performed to evaluate MSCs differentiation.RT-PCR was performed to analyze microRNA130α expression at different time points.Results microRNA130α was down-modulated during chondrogenesis after BMSCs been cultured with TGF-β1 for 7 days (P ＜0.05).Conclusion During the early stage of BMSC chondrogenic differentiation,mciroRNA130a expression was specifically repressed,suggesting its role in differentiation of rat bone mesenchymal stromal cells.
