Analysis of drug-resistant genes of 60 Acinetobacter baumannii isolates
- VernacularTitle:60株鲍曼不动杆菌耐药基因携带情况分析
- Author:
Yuping WANG
;
Yanrong SONG
;
Jihong LI
;
Aixiang LIU
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
multidrug-resistance;
β-lactamase gene;
class 1 integron gene
- From:
Basic & Clinical Medicine
2010;30(3):297-302
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate β-lactamase gene and class 1 integron gene from 60 clinical Acinetobacter baumannii isolates.Methods The minimal inhibitory concentrations (MICs) for 16 antibiotics widely used were determined using the standard broth microdilution method.The β-lactamase gene,class 1 integron gene and adeB gene were determined by PCR and then sequenced.Results Fifty-three strains of the 60 A.baumanii isolates were multi-drug resistant.OXA-23 gene was detected positive in six A.baumanii isolates,which were all resistant to more than five antimicrobial agents including carbapenem and showed high resistance to many antibiotics.Thirtyeight strains earring PER-1 gene showed higher resistance to cephalosporins than those without this gene (P＜0.01).Class 1 integron gene was positive in 45 strains,which exhibited significantly higher multiple resistance than those without this gene (P＜0.01).Twenty-five strains carrying both class 1 integron and PER-1 genes had a markedly higher multiple resistance (P＜0.01),but not in resistant level,compared to the 7 strains without these two genes.Conclusion Class 1 integron and β-lactamase gene may be the causes of muhidrug-resistance of A.baumanii.The strains carrying OXA-23 gene always showed multiple and high resistance to several antibiotics,so effective measures must be taken to control the epidemic of these strains.
