Detection and homology analysis of virulence genes in pandrug-resistant Pseudo-monas aeruginosa
- VernacularTitle:泛耐药铜绿假单胞菌的毒力基因检测和同源性分析
- Author:
Huiling CHEN
;
Hui SHENG
;
Huifen YE
;
Yingwei YANG
;
Yiyan YANG
;
Xiaoyuan HUANG
;
Yinmei YANG
- Publication Type:Journal Article
- Keywords:
pandrug-resistant;
Pseudomonas aeruginosa;
virulence gene;
BOX-PCR;
homology
- From:
Chinese Journal of Infection and Chemotherapy
2013;(6):469-472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the prevalence and sequence homology of virulence genes exoU and exoS in 53 strains of pan-drug-resistant Pseudomonas aeruginosa .Methods The virulence genes exoU and exoS were detected by PCR.Sequence homo-logy was analyzed by BOX-PCR.Results Of the 53 clinical isolates of pandrug-resistant Pseudomonas aeruginosa ,the exoS+/exoU- genotype was identified in 40 strains,exoU+/exoS - genotype in 10 strains,exoS +/exoU+ genotype in 1 strain, and exoS-/exoU- genotype in 2 strains.BOX-PCR results showed that 41 exoS+ isolates belonged to 24 genotypes,and 11 exoU+ strains could be grouped into 7 genotypes.Conclusions The prevalence of virulence genes is high in clinical isolates of pandrug-resistant Pseudomonas aeruginosa .BOX-PCR fingerprint analysis combined with sequence homology analysis is help-ful for effective monitoring and control of hospital pandrug-resistant pseudomonas aeruginosa infection.
