Discussion on Cell Apoptosis and Its Related Molecular Mechanisms of Human Gastric Carcinoma Cell Line BGC-823 Induced by Cinnamaldehyde
10.11842/wst.2013.05.025
- VernacularTitle:桂皮醛诱导人胃癌BGC-823细胞凋亡及相关分子机制的探讨
- Author:
Chengcheng FENG
;
Xi ZOU
;
Jian WU
;
Jinyong ZHOU
;
Ruiping WANG
- Publication Type:Journal Article
- Keywords:
Cinnamaldehyde;
human gastric carcinoma cell line BGC-823;
cell proliferation;
cell apoptosis
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(5):920-925
- CountryChina
- Language:Chinese
-
Abstract:
This article was aimed to investigate the cell proliferation , cell apoptosis and its related molecular mechanisms of the human gastric carcinoma cell line BGC-823 in v itro after treatment with cinnamaldehyde . The MTT Assay demonstrated the inhibitory effect of cinnamaldehyde . And the Flow Cytometry was used to determine its induction of cell apoptosis. The Hoechst 33342 was used to observe morphological changes during apoptosis . Moreover , quantitative real time PCR and western blot analysis were used to detect the effect of cinnamaldehyde on human gastric carcinoma cell line BGC-823 . The results showed that compared with the control group , cinnamaldehyde had inhibitory effect on human gastric carcinoma cell line BGC-823 ( P <0 . 01 ) . It showed that cinnamaldehyde induced apoptosis through the downregulation of Bcl-2 , Bcl-xL and Survivin expression , upregulation of Bax and Bak expression , downregulation of Bcl-2 and Procaspase-3 , and upregulation of BAX . It was concluded that cinnamaldehyde had inhibitory effect on the proliferation of human gastric carcinoma cell line BGC-823 and induced apoptosis . It may be related to the activation of the endogenous apoptosis pathway .
