Stromelysin-1 and Membrane type-MMP-1 Expressions in Human Chronic Periodontitis with Type 2 Diabetes Mellitus.
10.5051/jkape.2008.38.4.629
- Author:
Sang Ho RYU
1
;
Jin Woo PARK
;
Jo Young SUH
;
Jae Mok LEE
Author Information
1. Department of Periodontology, School of Dentistry, Kyungpook National University, Korea. leejm@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Stromelysin-1;
MT-MMP-1;
DM;
chronic periodontitis
- MeSH:
Adult;
Blotting, Western;
Bone Resorption;
Chronic Periodontitis;
Diabetes Mellitus, Type 2;
Fibrinolysin;
Gingiva;
Hemorrhage;
Humans;
Inflammation;
Matrix Metalloproteinase 14;
Membranes;
Periodontal Pocket;
Tooth Extraction
- From:The Journal of the Korean Academy of Periodontology
2008;38(4):629-638
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purposes of this study were to compare and quantify the expression of Stromelysin-1 and MT-MMP-1 in the gingival tissues of patients with type 2 diabetes mellitus(DM) and healthy adults with chronic periodontitis. MATERIALS AND METHODS: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was devided into three groups. Group 1 (n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from systemically healthy 8 patients. Group 2 (n=8) is inflammed gingiva from patients with chronic periodontitis. Group 3 (n=8) is inflammed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Western blotting. The quantification of Stromelysin-1 and MT-MMP-1 were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test. RESULTS: In the analysis of expression levels, Stromelysin-1 and MT-MMP-1 expressions were similar in group 1 and 2. Stromelysin-1 and MT-MMP-1 expressions was more increased in group 3 than group 1, 2. The difference between group 3 and group 1, 2 was statistically significant. Also, in the interrelationship of Stromelysin-1 and MT-MMP-1 expressions, expressions of Stromelysin-1 and MT-MMP-1 showed increasing tendency in chronic periodontitis associated with type 2 DM and it seems that the MT-MMP-1 expressions were increasing in proportion to Stromelysin-1 expressions. CONCLUSION: It is suggested that Stromelysin-1 and MT-MMP-1 may be partly involved in the progression of periodontal inflammation associated with type 2 DM, as related to a metabolism of other factors, such as AGE, plasmin and other inflammatory mediators. Therefore, the expression levels of Stromelysin-1 and MT-MMP-1 can be inflammatory markers of periodontal inflammed tissue with type 2 DM.
