Effect and mechanism of cell differentiation agent Ⅱ on the differentiation of HL-60 cells
10.3760/cma.j.issn.1006-9801.2013.06.001
- VernacularTitle:细胞分化剂Ⅱ诱导HL-60细胞的分化及其机制
- Author:
Guorong JIN
;
Xiumei LIN
;
Yanli XU
;
Weiwei LIU
;
Xin ZHOU
;
Yan TANG
;
Huangxuan SHEN
- Publication Type:Journal Article
- Keywords:
Cell differentiation agent Ⅱ;
Cell line,HL-60;
Cell differentiation;
Gene expression profiling
- From:
Cancer Research and Clinic
2013;(6):361-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of cell differentiation agent Ⅱ (CDA-Ⅱ) on the differentiation of human acute mycloid leukemia (APL) HL-60 cells.Methods The cell morphology and differentiation was detected by Wright-Giemsa staining,the expression of cell surface differentiation antigen CD11b of HL-60 was detected by flow cytometry,the differentially expressed genes were screened by gene expression profile chip (NimbleGen).Results The result of Wright-Giemsa staining showed that CDA-Ⅱ induced HL-60 differentiation towards mature stages in a time-dependent manner.After treated with CDA-Ⅱ,the percentage of CD11b-positive HL-60 cells significantly increased in a time-and dose-dependent manner.The result of gene expression profiling indicated differentially expressed genes including 113 up-regulation genes and 140 down-regulation genes.The up-regulation expression genes involved in six pathways including mineral absorption,complement and coagulation cascades,down-regulation expression genes involved in nine pathways including pyrimidine metabolism,RNA polymerase,purine metabolism and so on.Conclusion CDA-Ⅱ can induce HL-60 differentiation and make gene differentially expressed.The data provide the feasibility of CDA-Ⅱ in differentiation induction therapy for APL.
