"Influences of ""Antiphlogistic No.1"" on the degranultion of Activated RBL-2H3 cells"
10.3760/cma.j.issn.1673-4246.2013.07.010
- VernacularTitle:抗炎一号对活化嗜碱性白血病细胞脱颗粒的影响
- Author:
Xiaoying ZHANG
;
Ping XU
;
Huimin ZHANG
;
Bin LI
;
Jianxiu YU
- Publication Type:Journal Article
- Keywords:
"""Antiphlogistic No.1"";
RBL-2H3;
Degranultion;
β-hexosaminidase;
IL-4;
Dexamethasone"
- From:
International Journal of Traditional Chinese Medicine
2013;(7):607-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the direct effect ofAntiphlogistic No.1 on the degranultion of RBL-2H3 mast cells stimulated by anti-DNP IgE and DNP-BSA complex.Methods The effect of Antiphlogistic No.1 on stabilization of RBL-2H3 cell membrane was assessed by degranultion inhibition rate.β-hexosaminidase and IL-4 released from RBL-2H3 cells were detected by PNAG colorimetric assay and ELISA.Results β-hexosaminidase and cytokine IL-4 production releases from RBL-2H3 cells was reduced after they had been treated with Antiphlogistic No.1.The inhibition rate of β-hexosaminidase release was 42.47％ at the concentration of 8 μg/ml (P＜0.01) and dose dependently increased to 52.40％,68.26％ and 72.15％ respectively when drug concentration up to 80 μg/ml,800 μg/ml,8000 μg/ml,while inhibition rate of IL-4 generation were 13.87％,23.27％,31.95％,39.99％ (P＜0.01).Antiphlogistic No.1 maximum inhibition rate of β-hexosaminidase release is 84.48％ of dexamethason maximum inhibition rate while Antiphlogistic No.l maximum inhibition rate of IL-4 is close to dexamethason maximum inhibition rate.Conclusion Antiphlogistic No.1 could stabilize the cellular membrane of RBL-2H3 and provide protection against Ⅰ type allergic response.
