Elaboration of protocol in dosage of ferulic acid in Radix angelicae sinensis by HPLC
- Author:
Tran Huu Dung
- Publication Type:Journal Article
- Keywords:
Ferulic acid;
Radix angelicae sinensis;
HPLC method;
Elaboration
- MeSH:
Coumaric Acids;
Drugs;
Chinese Herbal
- From:Pharmaceutical Journal
2005;348(4):21-24
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The extracted and purified ferulic acid residue from Radix angelicae sinensis was quantified by an established HPLC system composed of the following conditions: reversed phase column C-18 Purosphere 4.6mm 1D x 250 mm (5mm); mobile phase: acetonitrile/potassium dihydrophosphate 10 mM/L (15/85); pH: 4.5; flow rate: 1.2 mL/min; column temperature: 30oC. The results showed that the method can meet the following criteria: Rs > 1.5, RSD < 2%, N > 1 000, Tf in the range 0.8-1.2
