A study on expression of tumor necrosis factor alpha in middle ear cholesteatoma and its effect on bone destruction
10.3969/j.issn.1001-1781.2001.02.008
- VernacularTitle:肿瘤坏死因子-α在中耳胆脂瘤的表达及其对邻近骨质的作用
- Author:
Zhengxian LI
;
Xuepei LI
;
Gengxun LIU
- Publication Type:Journal Article
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2001;(2):66-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the expression and localization of tumor necrosis factor alpha(TNF-α) in human middle ear cholesteatoma and its effects on bone resorption.Method:The distribution of TNF-α in 18 cholesteatoma tissues and 8 normal external ear canal skin, facial skin and tympanic memebrane were studied immunohistochemically with paraffin-embedded sections.Result:TNF-α was localized in the epitheliun and connective tissue of cholesteatomas studied, particularly on basal and spinous cells and macrophages.The epithelium of normal external ear canal skin , facial skin and tympanic membrane was slightly stained.Conclusion:This study revealed significantly increased level of TNF-α in cholesteatoma tissues.TNF-α acts both directly,by causing bone erosion as an autocrine growth factor,and indirectly as an important mediator by stimulating the release of enzymes causing bone destruction.
