Expression and effect of HSV-TK gene in osteosarcoma cells
10.3969/j.issn.1001-7399.2001.02.015
- VernacularTitle:单纯疱疹病毒胸苷激酶基因在骨肉瘤细胞中的表达和作用
- Author:
Hui QIAO
;
Jushi QIU
;
Yang LI
;
Huizhong ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Clinical and Experimental Pathology
2001;(2):143-145
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To observe the killing effect of HSV-TK/GCV system on osteosarcoma cells. Methods The eukaryotic exprssoin vector (tgCMV-HyTK) containing HSV-TK gene was transduced into human osteosarcoma cell lines SAOS-2 by DOTAP. Total RNA extracted from HSV-TK expressing cells (SAOS-2TK) were tested by RNA dot blot analysis. A chemosensitivity of SAOS-2TK cells to GCV and “bystander effect” were measured by means of MTT colrimetric assay. Results HSV-TK gene was expressed in SAOS-2TK cells. SAOS-2TK cells were susceptible to the cytotoxicity of GCV. A meaningful “bystander effect” was demonstrated. Conclusion HSV-TK/GCV system may kill selectively the human osteosarcoma cell line SAOS-2.
