Determination of astragaloside IV in the Huangqijing by HPLC-RI
- VernacularTitle:HPLC示差折光检测测定黄芪精口服液中黄芪甲苷的含量
- Author:
Yumei CHI
;
Haiying JIANG
- Publication Type:Journal Article
- From:
Chinese Pharmaceutical Journal
2001;(1):49-51
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a HPLC-RI assay for the determination of astragaloside IV in the Huangqijing solution.METHODS The column Hypersil ODS 2(4.6 mm×200 mm,5 μm) was used.The mobile phase was consisted of methanol ane water (67∶33).The flow rate was 1 mL.min-1.RESULTS The standard curve was linear over the range of 1～5 μg with the correlation coefficient 0.9999.The average recovery was 98.1% with the RSD 2.02%(n＝6).CONCLUSION This method was sensitive,accurate,repeatable and easy to operate.
