The influence of PTD-OD-HA fusion proteins on apoptosis of bcr/abl positive cell lines
10.3781/j.issn.1000-7431.2010.04.001
- VernacularTitle:PTD-OD-HA融合蛋白对bcr/abl阳性细胞凋亡的影响
- Author:
Zhenglan HUANG
;
Maosheng JI
;
Ying YUAN
;
Shifeng HUANG
;
Dingbin LIU
;
Jianming ZENG
;
Jianping WEN
;
Wenli FENG
- Publication Type:Journal Article
- Keywords:
Leukemia,myeloid,chronic;
Recombinant fusion proteins;
Apoptosis;
Fusion proteins,bcr-abl
- From:
Tumor
2010;(4):267-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of protein transduction domain (PTD)-oligomerization domain (OD)-HA fusion proteins on apoptosis of bcr/abl-positive cell lines. Methods:bcr/abl-positive cells were treated with PTD-OD-HA protein. The apoptoses of the cells were detected by flow cytometry (FCM), DNA ladder and transmission electron microscopy (TEM), and the levels of apoptosis-related genes bax and bcl-2 were detected by RT-PCR and Western blotting. Results:FCM examination demonstrated that PTD-OD-HA protein induced the apoptosis of bcr/abl-positive cells; DNA ladder showed that the classic DNA ladders appeared in BaF3-P210 and K562 cells after 48 h treatment with PTD-OD-HA proteins; the apoptoses of BaF3-P210 cells were observed by TEM; the levels of bax in mRNA and protein increased in BaF3-P210 and K562 cells, and bcl-2 decreased. Conclusion:PTD-OD-HA proteins specifically induced the apoptosis of bcr/abl positive cells.
