Stat3 antisense oligodeoxynucleotide induces apoptosis in laryngeal carcinoma cells by regulating apoptosis-related factors
10.3969/j.issn.1001-1781.2010.04.005
- VernacularTitle:Stat3反义核苷酸通过调节凋亡相关因子诱导喉癌细胞凋亡
- Author:
Haili Lü
;
Qiuhang ZHANG
;
Bo YAN
- Publication Type:Journal Article
- Keywords:
laryngeal neoplasms;
antisense oligodeoxynucleotide;
Stat3;
apoptosis mechanism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;(4):155-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of apoptosis in laryngeal carcinoma cell induced by Stat3 antisense oligodeoxynucleotide (ASODN).Method:The designed Stat3 ASODN was transferred into laryngeal cacinoma Hep-2 cell by lipofection. Expression of Bcl-2, Bax and C-Myc were detected by Western blot and PCR.Result:Western blot and PCR results demonstrated that Stat3 ASODN could significantly increased the expression of Bax and decreased the expression of Bcl-2 and C-Myc when the concentration of antisense oligodeoxynucleotide were heightened.Conclusion:Stat3 ASODN participate in apoptosis by enhancing the expression of Bax and reducing the expression of Bcl-2 and C-Myc.
