Extraction of polysaccharide from Poacynum Hoacynum leaves and component analysis
- VernacularTitle:大叶白麻叶多糖提取及组分分析
- Author:
Junyou SHI
;
Yourui SUO
;
Guoliang LI
;
Zhiwei SUN
;
Yongjun LIU
- Publication Type:Journal Article
- Keywords:
Poacynum hendersonii;
polysaccharide;
monosaccharide;
anthrone-H_2SO_4 colorimetry;
HPCE
- From:
Chinese Traditional Patent Medicine
2010;(1):102-106
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To extract and isolate polysaccharide from Poacynum Hendersonii leaves,determine its content and analyze the monosaccharide composition.METHODS: Poacynum Hendersonii leaves was extracted with hot water,crude polysaccharide was precipitated with ethanol,deproteinated according to Sevage method,coloured with acticarbon.Then of polysaccharide contents were measured by anthrone-H_2SO_4 colorimetry at the wavelength of 620 nm.The monosaccharide composition was determined by HPCE.RESULTS: The polysaccharide content was 0.97% of leaf weight,and Gal,Ara,and Man contents were three higher monosaccharides.CONCLUSION : The method is easy to carry out the baseline resolution in HPCE and has highly sensitivity.
