Effect of Xilei Powder on primary cultured fibroblast functions related to wound healing
- VernacularTitle:锡类散对原代培养的小鼠口腔成纤维细胞与创面愈合相关功能的影响
- Author:
Jianping DAI
;
Jun CHEN
;
Yufei BEI
;
Bangxing HAN
;
Xiaokun ZHOU
- Publication Type:Journal Article
- Keywords:
collagen synthesis;
fibroblast;
MMP-2,9;
TIMP-1;
wound healing
- From:
Chinese Traditional Patent Medicine
2010;(1):30-35
- CountryChina
- Language:Chinese
-
Abstract:
AIM:Xilei Powder,a traditional Chinese prescription,has been used to treat wounds for hundreds of years,but the mechanism has not been fully understood.METHODS:The effects of Xilei Powder on fibroblast proliferation,collagen accumulation,matrix metalloproteinases-2,9 ( MMP-2,9 ) activities and tissue inhibitor of metalloproteinase 1 (TIMP-1) production were investigated by MTT,chloramine T method,gelatin zymography and enzyme-linked immunosorbent assays ( ELISA),respectively.RESULTS: The aqueous extract of Xilei Powder significantly promoted fibroblasts proliferation in a time and concentration manner,the population doubling time (125 μg/mL) was 33.8 h,it also significantly (P ＜0.05 ) promoted collagen production.Both of the aqueous and alcoholic extracts could significantly ( P ＜ 0.05 ) increase MMPo2 activity,and also very significantly ( P ＜ 0.01 )promote TIMP-1 production.CONCLUSION: Xilei Powder could promote fibroblasts proliferation,collagen and TIMP-1 production,this might be parts of mechanism to promote wound healing.
