TIMP-3 regulated by miR-181b as a target gene on invasion and migration of hepatocellular carcinoma cells
10.3760/cma.j.issn.1007-8118.2013.01.010
- VernacularTitle:miR-181b调控靶基因TIMP-3对肝癌细胞侵袭迁移能力的影响
- Author:
Baohua ZHU
;
Yuanzhi LU
;
Jinkai YUAN
;
Kun WANG
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Gene expression regulation,neoplastic;
Neoplasm metastasis
- From:
Chinese Journal of Hepatobiliary Surgery
2013;(1):29-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of TIMP 3 regulated by miR-181b as a target gene on invasion and migration of hepatocellular carcinoma (HCC) in vitro.Methods The expressions of miR-181b were detected using SYBR Green real-time fluorescence quantitative polymerase chain reaction on liver cancer specimens and on HCC cell lines.The protein expression of TIMP 3 in HCC was detected using westen blot,and SKHep-1 as a cell line expressing high miR-181b was chosen through reporter gene experiment.TIMP-3 as a target gene regulated by miR-181b and its effect on invasion and migration treated by anti-miR-181 b were studied using transwell and cell scarification test,respectively.Results The expression of miR-181b in HCC was higher than cancer-adjacent tissues and normal liver tissues.The differences among them were significant.There was a correlation between the high expression of miR-181b and invasiveness and metastasis in HCC.The protein expression of TIMP-3 in HCC was significantly lower than normal liver tissues and cancer-adjacent tissues.Expression of miR-181b mRNA was detected in various HCC cell lines such as Hep3B,HepG2,Huh 7,SKHep-1,SNU182,SNU449 and hepatocyte,with the expression of miR-181b in SKHep-1 being the highest (P＜0.01).TIMP3-3UTR was low when the expression of miR-181b was high (P＜0.05).The invasion and migration abilities of SKHep-1 were significantly inhibited by anti-miR-181b (P＜0.05).Conclusion The data suggested that miR-181b promoted invasion and migration of SKHep-1 by down-regulating TIMP-3 in HCC.
