Platelet-Derived Growth Factor Receptor-Positive Pericytic Cells of White Adipose Tissue from Critical Limb Ischemia Patients Display Mesenchymal Stem Cell-Like Properties.
10.4055/cios.2017.9.2.239
- Author:
Eo Jin KIM
1
;
Sang Gyo SEO
;
Hyuk Soo SHIN
;
Doo Jae LEE
;
Ji Hye KIM
;
Dong Yeon LEE
Author Information
1. Department of Orthopedic Surgery, Hanil General Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Platelet-derived growth factor;
Adipose tissue;
Adult stem cells;
Ischemia
- MeSH:
Adipose Tissue;
Adipose Tissue, White*;
Adult Stem Cells;
Amputation;
Blood Vessels;
Extremities*;
Flow Cytometry;
Humans;
In Vitro Techniques;
Ischemia*;
Mesenchymal Stromal Cells;
Pericytes;
Platelet-Derived Growth Factor*;
Receptors, Platelet-Derived Growth Factor;
Stem Cells;
Subcutaneous Fat
- From:Clinics in Orthopedic Surgery
2017;9(2):239-248
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The pericytes in the blood vessel wall have recently been identified to be important in regulating vascular formation, stabilization, remodeling, and function. We isolated and identified pericyte-like platelet-derived growth factor receptor beta-positive (PDGFRβ+) cells from the stromal vascular fraction (SVF) of adipose tissue from critical limb ischemia (CLI) patients and investigated their potential as a reliable source of stem cells for cell-based therapy. METHODS: De-identified subcutaneous fat tissues were harvested after amputation in CLI patients. Freshly isolated SVF cells and culture-expanded adipose-derived stem cells (ADSCs) were quantified using flow cytometry. A matrigel tube formation assay and multi-lineage differentiation were performed to assess pericytic and mesenchymal stem cell (MSC)-like characteristics of PDGFRβ+ ADSCs. RESULTS: PDGFRβ+ cells were located in the pericytic area of various sizes of blood vessels and coexpressed mesenchymal stem cell markers. PDGFRβ+ cells in freshly isolated SVF cells expressed a higher level of stem cell markers (CD34 and CXCR4) and mesenchymal markers (CD13, CD44, CD54, and CD90) than PDGFRβ– cells. In vitro expansion of PDGFRβ+ cells resulted in enrichment of the perivascular mesenchymal stem-like (PDGFRβ+/CD90+/CD45–/CD31–) cell fractions. The Matrigel tube formation assay revealed that PDGFRβ+ cells were located in the peritubular area. CONCLUSIONS: PDGFRβ+ ADSCs cells demonstrated a good multilineage differentiation potential. Pericyte-like PDGFRβ+ cells from the SVF of adipose tissue from CLI patients had MSC-like characteristics and could be amplified by in vitro culture with preservation of their cell characteristics. We believe PDGFRβ+ cells in the SVF of adipose tissue can be used as a reliable source of stem cells even in CLI patients.
