Determination of myricetrin and quercitroside in euphorbia hirta L. by RP-HPLC
10.3760/cma.j.issn.1673-4246.2013.01.014
- VernacularTitle:反相高效液相色谱法同时测定飞扬草中杨梅苷和槲皮苷含量的方法探讨
- Author:
Yong ZENG
;
Xiaomei HU
;
Jinghong QU
- Publication Type:Journal Article
- Keywords:
Euphorbia hirta L.;
Myricetrin;
Quercitroside;
RP-HPLC
- From:
International Journal of Traditional Chinese Medicine
2013;(1):46-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Euphorbia Hirta L.Methods The ZORBAX SB-C18 (250 mm × 4.6 mm,5 μn) column was used,the mobile phase consisted of acetonitrile:0.1％ H3PO4(21 ∶ 79),the flow rate was 0.8 ml/min,the column temperature was 30℃ the detecting wavelength was at 256 nm.Results The cablibration curve was linear within a range of 0.013～0.26 mg/ml and 0.008～0.16 mg/ml,the average recovery was 99.1％,98.9％and the RSD was 0.91％,1.55％,respectively.Conclusion The method is simple,repeatable and accurate,it can be applied in quantitative determination of myricetrin and quercitroside in Euphorbia Hirta L..
