Influence of complement C1q on beta amyloid fibers-induced BV-2 microglia inflammation

VernacularTitle:补体C1q对Aβ纤维诱导的小胶质细胞炎性反应的影响
Author: Shuwen XU ; Xiahui ZHANG ; Dongfeng LI ; Baoping WANG ; Shaotong XIANG
Publication Type:Journal Article
Keywords: Alzheimer disease; Complement C1q; Amyloid beta-peptides; Microglia
From: Chinese Journal of Geriatrics 2013;(1):96-98
CountryChina
Language:Chinese
Abstract: Objective The impact of complement Clq on inflammation in beta amyloidstimulated microglia.Methods After the cultured BV-2 microglial cells were treated with 100mg/L beta-amyloid fibers (fAβs),some of them were given C1q,others wcrc given C1q and C1qA.Then,interleukin-6 (IL-6) and tumor necrosis factor α (TNF α) in the supernatant and cell lysate were determined by the sandwich ELISA.Results A significant increase in TNF-α started at giving 50 nmol/L C1q after 100 mg/L fAβs (F =1177.27,P＜ 0.05),while the release of TNF-α was significantly suppressed by using 50 nmol/L C1qA on basis of this(P＜0.05).The level of IL-6 showed no above change.Conclusions C1q may enhance the inflammation of Aβ-induced BV-2 microglia cells and TNF-α may play important role in this effect.