The effects of a high-intensity pulsed electromagnetic field on the expression of β-catenin in the neural stem cells of neonatal rats
10.3760/cma.j.issn.0254-1424.2012.010.003
- VernacularTitle:脉冲强磁场对新生大鼠神经干细胞β-连接素表达的影响
- Author:
Lili ZOU
;
Tao XU
;
Xinlan LONG
;
Lei SHI
;
Tao PENG
- Publication Type:Journal Article
- Keywords:
Electromagnetic stimulation;
Neural stem cells;
Cell proliferation;
Wnt/β-catenin
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2012;(10):729-732
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism by which a high-intensity pulsed electromagnetic field (HIPEMF) (0.1 Hz,4 T,8 pulses) facilitates the proliferation of neural stem cells by detecting the expression of β-catenin genes and protein.Methods Neural stem cells (NSCs) were isolated from the sub-ventricular zone (SVZ) of neonatal rats and cultured in supplemented,serum-free medium for two weeks.The NSCs were then divided into an experimental group exposed to a HIPEMF for 8 pulses and a control group given sham stimulation.The gene and prorein expression of β-catenin in the NSCs were assayed by RT-PCR and Western blotting on the 1st,3rd,5th and 7th day after the stimulation.Results The NSCs' cloned spheres were round and translucent,and showed red fluorescence after staining with anti-nestin (cy3).The RT-PCR results showed β-catenin genes were highly expressed in the exposed group (significantly more than in the controls).The Western blotting showed that expression of β-catenin protein was also higher in the experimental group,especially at the 7th day after stimulation,a difference which was also statistically significant Conclusion HIPEMF at 0.1 Hz,4 T,in 8 pulse trains can promote NSC proliferation,perhaps through the Wnt/β-catenin signaling pathways.
