A novel HLA allele, HLA-B * 40:74, detected in a Chinese individual
10.3760/cma.j.issn.0254-5101.2011.12.003
- VernacularTitle:中国人群新等位基因HLA-B*40:74的序列分析及认定
- Author:
Xiaofeng LI
;
Xu ZHANG
;
Kunlian ZHANG
;
Yang CHEN
;
Xianzhi LIU
;
Jianping LI
- Publication Type:Journal Article
- Keywords:
Allele;
HLA-B * 40∶74;
Sequencing-based typing
- From:
Chinese Journal of Microbiology and Immunology
2011;31(12):1068-1071
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify and confirm a novel HLA allele in a Chinese individual.MethodsA new HLA allele was found during routine HLA genotyping by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) and sequencing-based typing (SBT).ResultsThe new sequence differs from HLA-B * 40:01:01 by two nucleotide substitutions in exon 2 at positions 103 (G＞T) and 106 (A＞G) ; These mutations result in two codon changes:at codon 35 (GCC＞TCC) where an alanine (A) is substituted by a serine(S) and at codon 36(ATG＞GTG) where a methionine(M) is substituted by a valine (V).ConclusionA novel HLA allele,HLA-B * 40:74,was identified,and was named officially by the WHO Nomenclature Committee (HWS10004518 - EF458488).
