Effect of angiotensin 1-7 on human renal proximal tubular epithelial cell transdifferentiation induced by high glucose and its mechanism
10.3760/cma.j.issn.1001-7097.2011.12.008
- VernacularTitle:血管紧张素1-7对高糖诱导人肾小管上皮细胞转分化的影响及其机制
- Author:
Xiangyou LI
;
Guohua DING
;
Fengqi HU
;
Wei LIANG
;
Zhilong REN
- Publication Type:Journal Article
- Keywords:
Angiotensin-(l-7);
Peroxisome proliferator activated receptor-γ;
α-smooth muscle actin;
Transdifferentiation
- From:
Chinese Journal of Nephrology
2011;27(12):903-906
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of angiotensin 1-7(Ang 1-7) on renal proximal tubular epithelial cell(HK-2) transdifferentiation induced by high glucose.Methods All the raised HK-2 cells were divided into 5 groups: normal control group,high glucose group,high glucose with Ang1-7 group,high glucose with Ang1-7 and A779 group,high glucose with pioglitazone group.Expression of peroxisome proliferator activated receptor-γ(PPAR-γ) and α-smooth muscle actin(α-SMA) was detected by Western blotting,real-time PCR and immunofluorescence.Results The levels of PPAR-γ protein and mRNA in HK-2 cells were significantly increased after treatment with high glucose and Ang 1-7.Expression of α-SMA protein and mRNA was inhibited remarkably after treatment with high glucose and Ang 1-7.These effects of Ang 1-7 on HK-2 cells could be reversed by Mas receptor antagonist A779.Conclusion Ang 1-7 inhibits high glucose-induced expression of o-SMA in HK-2 cells,which is in part through the Mas.
